January 20, 2005

ACE inhibitor and angiotensin II type 1 receptor blocker differently regulate ventricular fibrosis in hypertensive diastolic heart failure

Structured PICO

Do ACE inhibitors and ARBs differently regulate ventricular fibrosis in a rat model of hypertensive diastolic heart failure?

Population

24 Dahl salt-sensitive rats (DHF model fed 8% NaCl diet from age 7 weeks, n=18; normal chow controls, n=6)

Intervention

Candesartan cilexetil (1 mg/kg per day) or temocapril hydrochloride (0.2 mg/kg per day) administered from age 8 weeks to 20 weeks

Comparator

Untreated DHF rats (fed 8% NaCl) and normal chow controls

Outcome

Ventricular fibrosis, type I collagen mRNA levels, and gelatinase activity at age 20 weekssurrogate

In a rat model of hypertensive diastolic heart failure, ARBs and ACE inhibitors inhibit ventricular fibrosis to a similar degree but through different mechanisms involving collagen synthesis and degradation.

Abstract

BACKGROUND: Promoted myocardial stiffening has a crucial role in the transition to overt diastolic heart failure (DHF) in hypertensive hearts and is attributed to progressive ventricular fibrosis. Previous studies revealed the effects of an angiotensin II type 1 receptor blocker (ARB) and an angiotensin-converting enzyme inhibitor (ACEI) on the synthesis and degradation of collagens in the other phenotype of heart failure, systolic heart failure, which has a different pathophysiology; however, little is known about their effects in DHF. OBJECTIVE: To investigate effects of an ACEI and an ARB on the regulatory system of ventricular fibrosis in hypertensive DHF. DESIGN AND METHODS: Dahl salt-sensitive rats fed a diet containing 8% NaCl from age 7 weeks (DHF model) were divided into three groups: six untreated rats, six rats treated with a subdepressor dose of an ARB, candesartan cilexetil (1 mg/kg per day), from age 8 weeks, and six rats treated with a subdepress or dose of an ACEI, temocapril hydrochloride (0.2 mg/kg per day), from age 8 weeks. Six Dahl salt-sensitive rats fed on normal chow served as controls. Data were collected when animals were aged 20 weeks. RESULTS: The administration of an ARB or an ACEI inhibited ventricular fibrosis to the same degree. The ACEI decreased the level of type I collagen mRNA, but the decrease was less than that induced by the ARB. The difference in collagen synthesis was probably cancelled out by that in degradation: both in-vitro and in-situ zymography showed that gelatinase activity was greater in the rats treated with the ACEI than in those treated with the ARB. CONCLUSIONS: An ARB and an ACEI inhibited ventricular fibrosis through different mechanisms in hypertensive DHF.

Mark Helpful

Bookmark

Relay