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Circadian rhythms are oscillations of physiology, behavior, and metabolism that have period lengths near 24 hours. In several model organisms and humans, circadian clock genes have been characterized and found to be transcription factors. Because of this, researchers have used microarrays to characterize global regulation of gene expression and algorithmic approaches to detect cycling. This article presents a new algorithm, JTKCYCLE, designed to efficiently identify and characterize cycling variables in large data sets. Compared with COSOPT and the Fisher's G test, two commonly used methods for detecting cycling transcripts, JTKCYCLE distinguishes between rhythmic and nonrhythmic transcripts more reliably and efficiently. JTKCYCLE's increased resistance to outliers results in considerably greater sensitivity and specificity. Moreover, JTKCYCLE accurately measures the period, phase, and amplitude of cycling transcripts, facilitating downstream analyses. Finally, JTKCYCLE is several orders of magnitude faster than COSOPT, making it ideal for large-scale data sets. JTKCYCLE was used to analyze legacy data sets including NIH3T3 cells, which have comparatively low amplitude oscillations. JTKCYCLE's improved power led to the identification of a novel cluster of RNA-interacting genes whose abundance is under clear circadian regulation. These data suggest that JTKCYCLE is an ideal tool for identifying and characterizing oscillations in genome-scale data sets.
Hughes et al. (Tue,) studied this question.