Further Observations on the Inhibition of Histamine Release by 2-Deoxyglucose

Chakravarty, N. Further observations on the inhibition of histamine release by 2-deoxyglucose. Acta physiol. scand. 1968. 72. 425–432. The effect of 2-deoxyglucose (2-DG) on histamine release from rat mast cells induced by compound 48/80 and antigen-antibody reaction has been studied. In a glucose-free oxygenated medium 2-DG has no effect on compound 48/80-induced histamine release and inhibits anaphylactic release only in high concentrations. Cyanide inhibition of both compound 48/80-induced and anaphylactic histamine release is however potentiated by a low concentration (5 mM) of 2-DG. When glucose is used to restore histamine release blocked by cyanide, 2-DG (5 mM) markedly inhibits the release. Iodoacetate, fluoride and oxamate have a similar blocking effect on the glucose-sustained histamine release in presence of cyanide. The findings are explained on the basis of a predominant inhibitory effect of 2-DG on the glycolytic pathway in rat mast cells.