Compared to light transmission aggregation, PFA-100 and VerifyNow assays showed sensitivities of 62.1% and 39.3% and specificities of 80.2% and 96.4% for detecting residual platelet reactivity.
Observational (n=484)
Do point-of-care methods (PFA-100 and VerifyNow) accurately detect residual platelet reactivity compared to light transmission aggregation in CAD patients undergoing PCI on dual antiplatelet therapy?
Point-of-care assays (PFA-100 and VerifyNow) correlate significantly with light transmission aggregation for detecting residual platelet reactivity in PCI patients, but exhibit varying sensitivity and specificity.
p-value: p=<.0001
Patients with coronary artery disease (CAD) receiving aspirin therapy with a residual platelet reactivity (RPR) may be at increased risk of ischemic vascular events. Point-of-care (POC) methods PFA-100 (Dade-Behring, Marburg, Germany) and VerifyNow (Accumetrics, San Diego, CA) assays have been suggested as rapid tools to evaluate RPR. We compared PFA-100 closure times by collagen/epinephrine and VerifyNow Aspirin assays with light transmission aggregation (LTA) induced by 1 mmol/L of arachidonic acid in 484 patients with CAD undergoing percutaneous coronary intervention and receiving dual antiplatelet therapy. RPR was detected in 30.0% of patients by LTA, in 32.4% by PFA-100, and in 14.3% by VerifyNow. Significant correlations were found among 3 methods (all P < .0001). In relation to the presence or absence of RPR by LTA and PFA-100, by LTA and VerifyNow, and by PFA-100 and VerifyNow, samples were significantly concordant (all P < .0001). Assuming LTA as the reference method, PFA-100 and VerifyNow showed sensitivity of 62.1% and 39.3% and specificity of 80.2% and 96.4%, respectively. The cutoff values for POC methods need to be defined for clinical use.
Paniccia et al. (Wed,) conducted a observational in Coronary artery disease (n=484). PFA-100 and VerifyNow assays vs. Light transmission aggregation (LTA) was evaluated on Detection of residual platelet reactivity (RPR) (p=<.0001). Compared to light transmission aggregation, PFA-100 and VerifyNow assays showed sensitivities of 62.1% and 39.3% and specificities of 80.2% and 96.4% for detecting residual platelet reactivity.