Dietary supplementation with nitrite prolonged bleeding time and inhibited platelet aggregation in mice, whereas dietary restriction of nitrite and nitrate enhanced platelet reactivity.
Does dietary manipulation of nitrite and nitrate levels alter platelet function in mice?
Endogenous levels of nitrite and nitrate, which can be manipulated by diet, inversely correlate with platelet reactivity and may serve as biomarkers for predicting platelet function.
p-value: p=<0.05
Nitric oxide (NO) appears to play an important role in the regulation of thrombosis and hemostasis by inhibiting platelet function. The discovery of NO generation by reduction of nitrite (NO₂⁻) and nitrate (NO₃⁻) in mammals has led to increased attention to these anions with respect to potential beneficial effects in cardiovascular diseases. We have previously shown that nitrite anions at 0.1 µM inhibit aggregation and activation of human platelet preparations in vitro in the presence of red blood cells and this effect was enhanced by deoxygenation, an effect likely due to NO generation. In the present study, we hypothesized that nitrite and nitrate derived from the diet could also alter platelet function upon their conversion to NO in vivo. To manipulate the levels of nitrite and nitrate in mouse blood, we used antibiotics, NOS inhibitors, low nitrite/nitrate (NOx) diets, endothelial NOS knock-out mice and also supplementation with high levels of nitrite or nitrate in the drinking water. We found that all of these perturbations affected nitrite and nitrate levels but that the lowest whole blood values were obtained by dietary restriction. Platelet aggregation and ATP release were measured in whole blood and the results show an inverse correlation between nitrite/nitrate levels and platelet activity in aggregation and ATP release. Furthermore, we demonstrated that nitrite-supplemented group has a prolonged bleeding time compared with control or low NOx diet group. These results show that diet restriction contributes greatly to blood nitrite and nitrate levels and that platelet reactivity can be significantly affected by these manipulations. Our study suggests that endogenous levels of nitrite and nitrate may be used as a biomarker for predicting platelet function and that dietary manipulation may affect thrombotic processes.
Park et al. (Fri,) conducted a other in Platelet Function and Hemostasis. Dietary nitrite and nitrate manipulation vs. Standard diet with normal water was evaluated on Platelet aggregation, ATP release, and bleeding time (p=<0.05). Dietary supplementation with nitrite prolonged bleeding time and inhibited platelet aggregation in mice, whereas dietary restriction of nitrite and nitrate enhanced platelet reactivity.