Key points are not available for this paper at this time.
At the beginning of biochemical work on male hormones it was assumed that the androgenic material which could be extracted from normal male urine was 'the male hormone', or 'the testicular hormone'. On this supposition, it was hoped that the biological assay of urine extracts would give an index of the activity of androgenic substances in the human body, possibly referable to the level of testicular secretion. It is for this reason that the separation of the maximal androgenic activity as measured by capon comb-growth has been taken as the criterion of a satisfactory extraction in the methods developed by systematic work in various laboratories. It has since been realized, however, that androgenic activity is not specific to one substance, and that at least two related compounds with androgenic activity are present in the mixture of neutral compounds which can be prepared from urine. Further, these two compounds, androsterone and
CALLOW et al. (Sun,) studied this question.