Inhibition of PKC by TPA or specific inhibitors resulted in decreased lipoprotein lipase synthesis by inhibition of translation, indicating a constitutive role of PKC in LPL gene expression.
Does PKC activation or inhibition regulate lipoprotein lipase synthesis in adipocytes?
Inhibition of PKC results in decreased LPL synthesis by inhibition of translation, indicating a constitutive role of PKC in LPL gene expression in adipocytes.
The hypertriglyceridemia of diabetes is accompanied by decreased lipoprotein lipase (LPL) activity in adipocytes. Although the mechanism for decreased LPL is not known, elevated glucose is known to increase diacylglycerol, which activates protein kinase C (PKC). To determine whether PKC is involved in the regulation of LPL, we studied the effect of 12-O-tetradecanoyl phorbol 13-acetate (TPA) on adipocytes. LPL activity was inhibited when TPA was added to cultures of 3T3-F442A and rat primary adipocytes. The inhibitory effect of TPA on LPL activity was observed after 6 h of treatment, and was observed at a concentration of 6 nM. 100 nM TPA yielded maximal (80%) inhibition of LPL. No stimulation of LPL occurred after short term addition of TPA to cultures. To determine whether TPA treatment of adipocytes decreased LPL synthesis, cells were labeled with 35Smethionine and LPL protein was immunoprecipitated. LPL synthetic rate decreased after 6 h of TPA treatment. Western blot analysis of cell lysates indicated a decrease in LPL mass after TPA treatment. Despite this decrease in LPL synthesis, there was no change in LPL mRNA in the TPA-treated cells. Long term treatment of cells with TPA is known to down-regulate PKC. To assess the involvement of the different PKC isoforms, Western blotting was performed. TPA treatment of 3T3-F442A adipocytes decreased PKC alpha, beta, delta, and epsilon isoforms, whereas PKC lambda, theta, zeta, micro, iota, and gamma remained unchanged or decreased minimally. To directly assess the effect of PKC inhibition, PKC inhibitors (calphostin C and staurosporine) were added to cultures. The PKC inhibitors inhibited LPL activity rapidly (within 60 min). Thus, activation of PKC did not increase LPL, but inhibition of PKC resulted in decreased LPL synthesis by inhibition of translation, indicating a constitutive role of PKC in LPL gene expression.
Ranganathan et al. (Mon,) reported a other. 12-O-tetradecanoyl phorbol 13-acetate (TPA) and PKC inhibitors was evaluated on Lipoprotein lipase (LPL) activity and synthesis. Inhibition of PKC by TPA or specific inhibitors resulted in decreased lipoprotein lipase synthesis by inhibition of translation, indicating a constitutive role of PKC in LPL gene expression.