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The usual transplantation procedure for determining the fraction of injected cells with colony-forming capability that actually settles in the spleen and there forms colonies is inadequate for computation of the number of colony-forming cells in a suspension from a count of spleen colonies. The use of f as a cellular index of transplantability may be questioned also. Circulation of cells appears to play the most significant role in determining the number of cells in the spleen during the first, hours after injection.
Kretchmar et al. (Sat,) studied this question.