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Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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Sabine Eckert
Francis Dziva
Roy R. Chaudhuri
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Journal of Bacteriology
University of Cambridge
University of Edinburgh
Wellcome Sanger Institute
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Eckert et al. (Sat,) studied this question.
www.synapsesocial.com/papers/69df17a2b8d7e94566614726 — DOI: https://doi.org/10.1128/jb.01292-10
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