Human Hepatocellular Carcinoma Cell Lines Secrete the Major Plasma Proteins and Hepatitis B Surface Antigen

Key Points

• The research aims to investigate the synthesis and secretion of major plasma proteins in human hepatoma cell lines, and their relevance to hepatitis B and tumorigenicity.
• Analysis of cell culture fluid from hepatoma-derived cell lines to identify synthesized proteins.
• Examination of Hep 3B cell line for hepatitis B surface antigen production.
• Injection of Hep 3B into athymic mice to monitor for metastatic tumors.
• Seventeen major human plasma proteins are synthesized and secreted by the new cell lines.
• The Hep 3B cell line produces the two major polypeptides of the hepatitis B surface antigen.
• Injection of Hep 3B into mice results in the development of metastatic hepatocellular carcinomas.

Abstract