Flexibility of the myosin heavy chain: direct evidence that the region containing SH1 and SH2 can move 10 .ANG. under the influence of nucleotide binding

Previous experiments demonstrated that two thiols of skeletal myosin subfragment 1 (SF1) could be oxidized to a disulfide bond by treatment with a 2-fold excess of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) in the presence of MgADP Wells, J. A., M. Elzinga, personal communication.(ABSTRACT TRUNCATED AT 250 WORDS)