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Antibodies to grass pollen allergens have been demonstrated on basophils of individuals with hay fever by means of a rosette-forming reaction with allergen-coated erythrocytes. The antibodies on the basophil cell membrane were shown to be IgE by inhibition of rosette-formation with specific anti-Ε serum. Not all persons giving positive skin reactions to grass pollen extracts had basophils capable of rosette-formation.
Wilson et al. (2009) studied this question.