Mutations in MTO1 cause isolated hypertrophic cardiomyopathy and lactic acidosis by impairing mtDNA translation and mitochondrial respiration.
Case Report (n=3)
Do mutations in the MTO1 gene cause hypertrophic cardiomyopathy and lactic acidosis by impairing mitochondrial respiration?
Mutations in the MTO1 gene cause hypertrophic cardiomyopathy and lactic acidosis by impairing mitochondrial translation and respiration.
Dysfunction of mitochondrial respiration is an increasingly recognized cause of isolated hypertrophic cardiomyopathy. To gain insight into the genetic origin of this condition, we used next-generation exome sequencing to identify mutations in MTO1, which encodes mitochondrial translation optimization 1. Two affected siblings carried a maternal c.1858dup (p.Arg620Lysfs(∗)8) frameshift and a paternal c.1282G>A (p.Ala428Thr) missense mutation. A third unrelated individual was homozygous for the latter change. In both humans and yeast, MTO1 increases the accuracy and efficiency of mtDNA translation by catalyzing the 5-carboxymethylaminomethylation of the wobble uridine base in three mitochondrial tRNAs (mt-tRNAs). Accordingly, mutant muscle and fibroblasts showed variably combined reduction in mtDNA-dependent respiratory chain activities. Reduced respiration in mutant cells was corrected by expressing a wild-type MTO1 cDNA. Conversely, defective respiration of a yeast mto1Δ strain failed to be corrected by an Mto1(Pro622∗) variant, equivalent to human MTO1(Arg620Lysfs∗8), whereas incomplete correction was achieved by an Mto1(Ala431Thr) variant, corresponding to human MTO1(Ala428Thr). The respiratory yeast phenotype was dramatically worsened in stress conditions and in the presence of a paromomycin-resistant (P(R)) mitochondrial rRNA mutation. Lastly, in vivo mtDNA translation was impaired in the mutant yeast strains.
Ghezzi et al. (Thu,) conducted a case report in Hypertrophic cardiomyopathy and lactic acidosis (n=3). MTO1 mutations vs. Wild-type MTO1 was evaluated on Identification of genetic mutations and functional impact on mitochondrial respiration. Mutations in MTO1 cause isolated hypertrophic cardiomyopathy and lactic acidosis by impairing mtDNA translation and mitochondrial respiration.