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A movable quartz micropipette, whose tip is sealed with a polymer plug, is used as a liquid-vacuum interface to a mass spectrometer. A light microscope allows observation of, and positioning of, the micropipette tip on the surface of a sample mounted in a perfusion chamber. This forms the basis of an instrument which enables one to study, in vitro, the localization of transepithelial transport of water and other molecules. Some preliminary results from the use of this instrument are presented.
Jarrell et al. (Fri,) studied this question.