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Mycoplasmas were isolated from 73 of 379 (19%) commercial bovine serum lots examined, including 55 of 239 (23%) unprocessed fetal bovine and calf serum sublots received from 4 suppliers and 18 of 140 (13%) final lots obtained from 2 of 5 manufacturers. Of 31 mycoplasma strains examined for identity, 28 were identified as M. arginini and 3 strains were unrelated to 39 well established Mycoplasma species and serotypes and may represent new species. Mycoplasma arginini was isolated from 10 uninoculated cell cultures and one virus pool also. Data presented indicate that M. arginini is a common contaminant of commercial bovine sera and of cell cultures and that bovine serum is a major source of bovine mycoplasma contamination of cell cultures.
Barile et al. (Mon,) studied this question.