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The extent of ATP synthesis from ADP and Pi at the active centre of myosin subfragment 1 has been reinvestigated. The results have been interpreted using a treatment which is not dependent on the number or nature of the intermediates in the ATPase mechanism. An average value for the binding constant of ATP of (3.25 +/- 0.96) X 10(11) M-1 at pH 8.0 23 degrees C and ionic strength 0.12 M was obtained. Additional evidence is given to confirm that synthesis at the active site has been investigated.
Goody et al. (Thu,) studied this question.
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