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Content of crude protein and cell wall constituents in leaves and stems of alfalfa (Medicago sativa L.) cv K-22 with advancing maturity was investigated. Sampling was done in 7-day intervals in second cut. During growth and development, crude protein content decreased in leaves and stems, from 332,6 to 328,8 gkg-1DM and from 138,6 to 122,0 gkg-1DM, respectively. From first to third development stage, content of ADF in leaves and stems increased, from 352,0 to 476,7 gkg-1DM and from 592,8 to 867,1 gkg-1DM, respectively. Highest contents of ADF and hemicellulose was stated in plant parts in third development stage, in leaves 187,4 and 289,3 gkg-1DM, and in stems 499,4 and 367,7 gkg-1DM. The highest changes of lignin contents are in stems, while content of lignin in leaves increased from 42,0 to 49,0 gkg-1DM.
Marković et al. (Mon,) studied this question.