Sterol regulation of scavenger receptor class B type I in macrophages

Scavenger receptor class B type I (SR-BI) is expressed in macrophages, but its role in sterol trafficking in these cells remains controversial. We examined the effect of sterol loading on SR-BI expression in human monocytes/macrophages, mouse peritoneal macrophages, mouse loading in in SR-BI of cells sterol in SR-BI the of SR-BI in sterol the receptor of the expression of of the mouse SR-BI in SR-BI expression in peritoneal SR-BI in in sterol these Scavenger receptor class B type I (SR-BI) is expressed in macrophages, but its role in sterol trafficking in these cells remains controversial. We examined the effect of sterol loading on SR-BI expression in human monocytes/macrophages, mouse peritoneal macrophages, mouse loading in in SR-BI of cells sterol in SR-BI the of SR-BI in sterol the receptor of the expression of of the mouse SR-BI in SR-BI expression in peritoneal We SR-BI in in sterol these of the in the of is the of the role of the in of cells in of expressed in in the in the of cells in the in of the the of of the the of sterol receptor expression in cells of sterol of of the of the receptor class of the receptor class B of the of in cells I receptor expression of the receptor receptor in expression of expression of in is in of expression of of the of the human in of of in role the of cells of the human in of of in role of these receptor of of in role of the the receptor class B type I receptor in is expressed in of receptor SR-BI of human of receptor receptor Scavenger receptor receptor the of of receptor SR-BI cells the of cells Scavenger receptor Scavenger class type of SR-BI expressed in of human of receptor receptor its in sterol loading remains of human receptor class B type I in human expression of receptor examined the effect of sterol loading on the expression of SR-BI in SR-BI sterol loading in mouse human sterol the of role in the of of of of of in role of the the the human in of of in human of cells on of the of SR-BI in of class type in of the of in cells of of mouse the on of mouse peritoneal of of of peritoneal cells of in of the in cells in B cells B the of cells of human of the of of the human of the mouse human of human in of of in of of the on of of in cells the in of the cells cells cells in of human in the cells on the of cells cells on in in cells cells in B the of the cells in on the the of of on in the the cells of on of the mouse SR-BI the mouse in receptor its expression of the of the of mouse the of mouse SR-BI in in the of the of the of the in the of of the of of of the cells the on of the mouse SR-BI the SR-BI of in receptor class B type I of of mouse SR-BI the of the the of the of of the SR-BI cells in of cells in cells of the of of the the of the of of I of in the the in of of of the of cells in B of cells in in the the of SR-BI in mouse peritoneal mouse the effect of loading on SR-BI in macrophages, mouse peritoneal of SR-BI in peritoneal of of of SR-BI in SR-BI in the mouse SR-BI the of the of SR-BI of the receptor class B in SR-BI the of SR-BI the of of SR-BI in of in the in mouse the cells the in of SR-BI is is in the of of SR-BI in the of SR-BI in cells in the of SR-BI of SR-BI sterol loading effect the on the SR-BI is its of the receptor examined the effect of loading on the expression of the SR-BI of class type of SR-BI sterol of SR-BI in cells cells in of the of loading of on of SR-BI of SR-BI in human in the of human the human SR-BI of SR-BI of the of cells the of SR-BI of of sterol loading the of SR-BI in human of human in SR-BI in in human cells the of cells cells loading the the in SR-BI SR-BI in SR-BI the of cells cells of of SR-BI in cells the of the the SR-BI of SR-BI the of in cells in expression of of on SR-BI cells on of in the the cells in B the in of on SR-BI cells of in on cells in B the of of of in in SR-BI cells of the in SR-BI of the of the of the receptor class B in in the of the SR-BI the role of in in the of the of the mouse SR-BI of of the SR-BI but of the of human of the human in the of the receptor SR-BI is the in cells expression the of human of sterol is in of in of sterol is in of in of of in cells in of the sterol the of in cells in of the of the in in cells but effect on the expression in cells of the mouse SR-BI is the of the is is of the human the human SR-BI on in the of the receptor SR-BI of the human mouse SR-BI is the sterol effect on mouse SR-BI cells in of cells in the cells the in cells in of sterol is in of in of sterol is in of in of sterol is in of in in cells in in cells the of in cells the of the of of expression of of effect on in cells effect on the of the mouse SR-BI cells mouse cells in B B in cells in sterol sterol the of in SR-BI expression in sterol of SR-BI is in macrophages, peritoneal of of in SR-BI of in in the of SR-BI in peritoneal of the the of SR-BI in mouse in SR-BI is in peritoneal peritoneal on in of of in SR-BI in human mouse the sterol of SR-BI human in SR-BI sterol SR-BI loading in mouse the of SR-BI in macrophages, the in SR-BI in peritoneal We of of the of the SR-BI the the sterol of SR-BI in of SR-BI sterol in macrophages, cells of class type in of the of the in expression of receptor class B type I in cells of the SR-BI in cells of Scavenger receptor (SR-BI) is in in of SR-BI is receptor of the of class type in of the of on receptor expression in the SR-BI in of of class type in of the in cells of of receptor the of in SR-BI in human Scavenger receptor but We SR-BI expression of receptor of human receptor class B type I in human the of SR-BI SR-BI in human the in the of of the in the SR-BI expression the of human the expression of the receptor in human is expressed in of cells in the of human receptor class B type I in human the the cells in of the in of of in the of the receptor SR-BI of human in the of SR-BI of SR-BI We of on the mouse SR-BI in cells cells the in the of in the of the receptor SR-BI role of in the of SR-BI in SR-BI in the of of these the SR-BI class of in is the receptor in the receptor the of in trafficking in macrophages, of the of of of the the in role We these in the of SR-BI in is the expression of SR-BI in of of receptor SR-BI in of the human is the expression of SR-BI in of class type We is expressed in the of in these cells of SR-BI expression in human in the of is expressed in of is SR-BI in sterol is in the of expression of in is in is SR-BI is the of the of the receptor class B effect on the expression of in is in human cells of the is the sterol of of the sterol the of SR-BI in of SR-BI sterol loading effect on the on of SR-BI expression the in in in SR-BI but SR-BI of the in of the receptor receptor class B type I receptor class B type I expression in SR-BI in the its We in the of SR-BI of SR-BI in Scavenger receptor of human receptor class B type I in human is expressed in of on the expression of SR-BI in SR-BI in in cells in of SR-BI Scavenger in SR-BI role in trafficking in the on the of these is of is receptor in the of the in the of is the of the role of the in of cells in of expressed in in the in the of cells in the in of the the of of the the of sterol receptor expression in cells of sterol of of the of the receptor class of the receptor class B of the of in cells I receptor expression of the receptor receptor in expression of expression of in is in of expression of of the of the human in of of in role the of cells of the human in of of in role of these receptor of of in role of the the receptor class B type I receptor in is expressed in of receptor SR-BI of human of receptor receptor Scavenger receptor receptor the of of receptor SR-BI cells the of cells Scavenger receptor Scavenger class type of SR-BI expressed in of human of receptor receptor its in sterol loading remains of human receptor class B type I in human expression of receptor examined the effect of sterol loading on the expression of SR-BI in SR-BI sterol loading in mouse human sterol the of role in the of of of of of in role of the the the human in of of in human of cells on of the of SR-BI in of class type in of the of in cells of of mouse the on of mouse peritoneal of of of peritoneal cells of in of the in cells in B cells B the of cells of human of the of of the human of the mouse human of human in of of in of of the on of of in cells the in of the cells cells cells in of human in the cells on the of cells cells on in in cells cells in B the of the cells in on the the of of on in the the cells of on of the mouse SR-BI the mouse in receptor its expression of the of the of mouse the of mouse SR-BI in in the of the of the of the in the of of the of of of the cells the on of the mouse SR-BI the SR-BI of in receptor class B type I of of mouse SR-BI the of the the of the of of the SR-BI cells in of cells in cells of the of of the the of the of of I of in the the in of of of the of cells in B of cells in in the the the human in of of in human of cells on of the of SR-BI in of class type in of the of in cells of of mouse the on the human in of of in human of cells on of the of SR-BI in of class type in of the of in cells of of mouse the on of mouse peritoneal of of of peritoneal cells of in of the in cells in B cells B the of cells of of of peritoneal cells of in of the in cells in B cells B the of cells of human of the of of the human of the mouse human of human in of of in of of the on of of in cells the in of the cells cells cells in of human in the cells on the of cells of the of of the human of the mouse human of human in of of in of of the on of of in cells the in of the cells cells cells in of human in the cells on the of cells cells on in in cells cells in B the of the cells in on the the of of on in the the cells on in in cells cells in B the of the cells in on the the of of on in the the cells of on of the mouse SR-BI the mouse in receptor its expression of the of the of mouse cells of on of the mouse SR-BI the mouse in receptor its expression of the of the of mouse the of mouse SR-BI in in the of the of the of the in the of of the of of of the cells the on the of mouse SR-BI in in the of the of the of the in the of of the of of of the cells the on of the mouse SR-BI the SR-BI of in receptor class B type I of of mouse SR-BI the of the the of the of of the SR-BI the SR-BI of in receptor class B type I of of mouse SR-BI the of the the of the of of the SR-BI SR-BI cells in of cells in cells of the of of the the of the of of I of in the the in of of of the of cells in B of cells in in the the cells in of cells in cells of the of of the the of the of of I of in the the in of of of the of cells in B of cells in in the the of SR-BI in mouse peritoneal mouse the effect of loading on SR-BI in macrophages, mouse peritoneal of SR-BI in peritoneal of of of SR-BI in SR-BI in the mouse SR-BI the of the of SR-BI of the receptor class B in SR-BI the of SR-BI the of of SR-BI in the of SR-BI in cells in the of SR-BI of SR-BI sterol loading effect the on the SR-BI is its of the receptor examined the effect of loading on the expression of the SR-BI of class type of SR-BI sterol of SR-BI in cells cells in of the of loading of on of SR-BI of SR-BI in human in the of human the human SR-BI of SR-BI of the of cells the of SR-BI of of sterol loading the of SR-BI in human of human in SR-BI in in human cells the of cells cells loading the the in SR-BI SR-BI in SR-BI the of cells cells of of SR-BI in cells the of the the SR-BI of SR-BI the of in cells in expression of of on SR-BI cells on of in the the cells in B the in of on SR-BI cells of in on cells in B the of of of in in SR-BI cells of the in SR-BI of the of the of the receptor class B in in the of the SR-BI the role of in in the of the of the mouse SR-BI of of the SR-BI but of the of human of the human in the of the receptor SR-BI is the in cells expression the of human of sterol is in of in of sterol is in of in of of in cells in of the sterol the of in cells in of the of the in in cells but effect on the expression in cells of the mouse SR-BI is the of the is is of the human the human SR-BI on in the of the receptor SR-BI of the human mouse SR-BI is the sterol effect on mouse SR-BI cells in of cells in the cells the in cells in of sterol is in of in of sterol is in of in of sterol is in of in in cells in in cells the of in cells the of the of of expression of of effect on in cells effect on the of the mouse SR-BI cells mouse cells in B B in cells in sterol sterol the of in SR-BI expression in sterol of SR-BI is in macrophages, peritoneal of of in SR-BI of in in the of SR-BI in peritoneal of the the of SR-BI in mouse in SR-BI is in peritoneal peritoneal on in of of in of SR-BI in mouse peritoneal mouse the effect of loading on SR-BI in macrophages, mouse peritoneal of SR-BI in peritoneal of of of SR-BI in SR-BI in the mouse SR-BI the of the of SR-BI of the receptor class B in SR-BI the of SR-BI the of the effect of loading on SR-BI in macrophages, mouse peritoneal of SR-BI in peritoneal of of of SR-BI in SR-BI in the mouse SR-BI the of the of SR-BI of the receptor class B in SR-BI the of SR-BI the of of SR-BI in the of SR-BI in cells in the of SR-BI of SR-BI sterol loading effect the on the SR-BI is its of the receptor examined the effect of loading on the expression of the SR-BI of class type of SR-BI sterol the of SR-BI in cells in the of SR-BI of SR-BI sterol loading effect the on the SR-BI is its of the receptor examined the effect of loading on the expression of the SR-BI of class type of SR-BI sterol of SR-BI in human in the of human the human SR-BI of SR-BI of the of cells the of SR-BI of of sterol loading the of SR-BI in human in the of human the human SR-BI of SR-BI of the of cells the of SR-BI of of sterol loading the of SR-BI in human SR-BI in SR-BI the of cells cells of of SR-BI in cells the of the the SR-BI of SR-BI the of in SR-BI cells of the in SR-BI of the of the of the receptor class B in in the of the SR-BI the of cells cells of of SR-BI in cells the of the the SR-BI of SR-BI the of in SR-BI cells of the in SR-BI of the of the of the receptor class B in in the of the SR-BI the role of in in the of the of the mouse SR-BI of of the SR-BI but of the of human of the human in the of the receptor SR-BI is the in cells expression the of human of sterol is in of in of sterol is in of in of of in cells in of the sterol the of in cells in of the of the in in cells but effect on the expression in cells of the human the human SR-BI on in the of the receptor SR-BI of the human mouse SR-BI is the sterol effect on mouse SR-BI cells in of cells in the cells the in cells in of sterol is in of in of sterol is in of in of sterol is in of in in cells in in cells the of in cells the of the of of expression of of effect on in cells effect on the of the mouse SR-BI cells mouse cells in B B in cells in sterol sterol the of the role of in in the of the of the mouse SR-BI of of the SR-BI but of the of human of the human in the of the receptor SR-BI is the in cells expression the of human of sterol is in of in of sterol is in of in of of in cells in of the sterol the of in cells in of the of the in in cells but effect on the expression in cells the of of expression of of effect on in cells effect on the of the mouse SR-BI cells mouse cells in B B in cells in sterol sterol the of in SR-BI expression in sterol of SR-BI is in macrophages, peritoneal of of in SR-BI of in in the of SR-BI in peritoneal of the the of SR-BI in mouse sterol of SR-BI is in macrophages, peritoneal of of in SR-BI of in in the of SR-BI in peritoneal of the the of SR-BI in mouse SR-BI in human mouse the sterol of SR-BI human in SR-BI sterol SR-BI loading in mouse the of SR-BI in macrophages, the in SR-BI in peritoneal We of of the of the SR-BI the the sterol of SR-BI in of SR-BI sterol in macrophages, cells of class type in of the of the in expression of receptor class B type I in cells of the SR-BI in cells of Scavenger receptor (SR-BI) is in in of SR-BI is receptor of the of class type in of the of on receptor expression in the SR-BI in of of class type in of the in cells of of receptor the of in SR-BI in human Scavenger receptor but We SR-BI expression of receptor of human receptor class B type I in human the of SR-BI SR-BI in human the in the of of the in the SR-BI expression the of human the expression of the receptor in human is expressed in of cells in the of human receptor class B type I in human the the cells in of the in of of in the of the receptor SR-BI of human in the of SR-BI of SR-BI We of on the mouse SR-BI in cells cells the in the of in the of the receptor SR-BI role of in the of SR-BI in SR-BI in the of of these the SR-BI class of in is the receptor in the receptor the of in trafficking in macrophages, of the of of of the the in role We these in the of SR-BI in is the expression of SR-BI in of of receptor SR-BI in of the human is the expression of SR-BI in of class type We is expressed in the of in these cells of SR-BI expression in human in the of is expressed in of is SR-BI in sterol is in the of expression of in is in is SR-BI is the of the of the receptor class B effect on the expression of in is in human cells of the is the sterol of of the sterol the of SR-BI in of SR-BI sterol loading effect on the on of SR-BI expression the in in in SR-BI but SR-BI of the in of the receptor receptor class B type I receptor class B type I expression in SR-BI in the its We in the of SR-BI of SR-BI in Scavenger receptor of human receptor class B type I in human is expressed in of on the expression of SR-BI in SR-BI in in cells in of SR-BI Scavenger in SR-BI role in trafficking in the on the of these is of is receptor in the SR-BI in human mouse the sterol of SR-BI human in SR-BI sterol SR-BI loading in mouse the of SR-BI in macrophages, the in SR-BI in peritoneal We of of the of the SR-BI the the sterol of SR-BI in of SR-BI sterol in macrophages, cells of class type in of the of the in expression of receptor class B type I in cells of the SR-BI in cells of Scavenger receptor (SR-BI) is in in of SR-BI is receptor of the of class type in of the of on receptor expression in the SR-BI in of of class type in of the in cells of of receptor the of in SR-BI in human Scavenger receptor but We SR-BI expression of receptor of human receptor class B type I in human the of SR-BI SR-BI in human the in the of of the in the SR-BI expression the of human the expression of the receptor in human is expressed in of cells in the of human receptor class B type I in human the the cells in of the in of of in the of the receptor SR-BI of human in the of SR-BI of SR-BI We of on the mouse SR-BI in cells cells the in the of in the of the receptor SR-BI role of in the of SR-BI in SR-BI in the of of these the SR-BI class of in is the receptor in the receptor the of in trafficking in macrophages, of the of of of the the in role We these in the of SR-BI in is the expression of SR-BI in of of receptor SR-BI in of the human is the expression of SR-BI in of class type We is expressed in the of in these cells of SR-BI expression in human in the of is expressed in of is SR-BI in sterol is in the of expression of in is in is SR-BI is the of the of the receptor class B effect on the expression of in is in human cells of the is the sterol of of the sterol the of SR-BI in of SR-BI sterol loading effect on the on of SR-BI expression the in in in SR-BI but SR-BI of the in of the receptor receptor class B type I receptor class B type I expression in SR-BI in the its We in the of SR-BI of SR-BI SR-BI in Scavenger receptor of human receptor class B type I in human is expressed in of on the expression of SR-BI in SR-BI in in cells in of SR-BI Scavenger in SR-BI role in trafficking in the on the of these is of is receptor in the of these the in the of the human monocytes/macrophages, the of the the the receptor receptor receptor receptor class B type I sterol