What question did this study set out to answer?

The study aims to measure the variation in ribosomal DNA copy number across different yeast genera.

January 1, 1993

Yeasts have a four‐fold variation in ribosomal DNA copy number

RMRyszard MaleszkaAustralian National University GCG. D. Clark‐WalkerAustralian National University

Key Points

The study aims to measure the variation in ribosomal DNA copy number across different yeast genera.
Used pulsed-field gel electrophoresis to analyze the rDNA cluster size.
Examined wild-type strains from genera including Candida, Kluyveromyces, and others.
Observed a four-fold variation in rDNA repeat numbers among species.
Identified P. tannophilus with the fewest repeats (28) and C. glabrata and S. pombe with over 115.
Found rDNA clusters represented by two loci in some species on one or two chromosomes.

Abstract

By employing pulsed-field gel electrophoresis we have determined the size of the rDNA cluster in wild-type yeast strains representing genera of Candida, Kluyveromyces, Pachysolen, Schizosaccharomyces and Torulaspora. Although the genome size of the examined species is similar (12.3-13.9 Mb), at least a four-fold variation has been observed between the lowest amount of rDNA repeats in P. tannophilus (28) and the highest in C. glabrata and S. poombe (> 115). In two species the rDNA cluster is represented by two loci, residing either in one (S. pombe) or two chromosomes (C. glabrata).

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