High cell density upregulated myogenic differentiation markers and negative regulating cell-cycle markers like CDKI p21 in a density-dependent manner in C2C12 cells.
INTRODUCTION: Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction. METHODS: To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities. RESULTS: We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKI p21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner. CONCLUSIONS: These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion.
Tanaka et al. (Fri,) conducted a other in Myogenesis. High cell density vs. Low cell density was evaluated on Myogenic differentiation marker expression. High cell density upregulated myogenic differentiation markers and negative regulating cell-cycle markers like CDKI p21 in a density-dependent manner in C2C12 cells.