In cultured neonatal rat cardiomyocytes, ANG II-induced hypertrophy was prevented by treatment with losartan or transfection with an MR-1 RNA interference plasmid.
MR-1 is upregulated in hypertrophied myocardium and its knockdown prevents ANG II-induced hypertrophy in vitro, suggesting it plays a role in cardiac hypertrophy.
p-value: p=<0.01
Myofibrillogenesis regulator-1 (MR-1) is a novel homologous gene, identified from a human skeletal muscle cDNA library, that interacts with contractile proteins and exists in human myocardial myofibrils. The present study investigated MR-1 protein expression in hypertrophied myocardium and MR-1 involvement in cardiac hypertrophy. Cardiac hypertrophy was induced by abdominal aortic stenosis (AAS) in Sprague-Dawley rats. Left ventricular (LV) hypertrophy was assessed by the ratio of LV wet weight to whole heart weight (LV/HW) or LV weight to body weight (LV/BW). Rat MR-1 (rMR-1) expression in the myocardium was detected by immunohistochemical and Western blotting analysis. Hypertrophy was induced by ANG II incubation in cultured neonatal rat cardiomyocytes. The effect of rMR-1 RNA interference on ANG II-induced hypertrophy was studied by transfection of cardiomyocytes with an RNA interference plasmid, pSi-1, which targets rMR-1. Hypertrophy in cardiomyocytes was assessed by 3HLeu incorporation and myocyte size. rMR-1 protein expression in cardiomyocytes was detected by Western blotting. We found that AAS resulted in a significant increase in LV/HW and LV/BW: 89% and 86%, respectively (P < 0.01). Immunohistochemistry and Western blot analysis demonstrated upregulated rMR-1 protein expression in hypertrophic myocardium. ANG II induced a 24% increase in 3HLeu incorporation and a 65.8% increase in cell size compared with control cardiomyocytes (P < 0.01), which was prevented by treatment with losartan, an angiotensin (AT1) receptor inhibitor, or transfection with pSi-1. rMR-1 expression increased in ANG II-induced hypertrophied cardiomyocytes, and pSi-1 transfection abolished the upregulation. These findings suggest that MR-1 is associated with cardiac hypertrophy in rats in vivo and in vitro.
Liu et al. (Sat,) conducted a other in Cardiac hypertrophy. MR-1 RNA interference (pSi-1) or losartan vs. Control cardiomyocytes was evaluated on Hypertrophy assessed by [3H]Leu incorporation and myocyte size (p=<0.01). In cultured neonatal rat cardiomyocytes, ANG II-induced hypertrophy was prevented by treatment with losartan or transfection with an MR-1 RNA interference plasmid.