Platelets from female mice demonstrated intrinsically greater reactivity, including increased fibrinogen binding and aggregation in response to agonists, compared with platelets from male mice.
Are platelets from female mice intrinsically more reactive to agonists than platelets from male mice?
Female mouse platelets are intrinsically more reactive to agonists than male platelets, which may provide mechanistic insight into sex differences in ischemic coronary events.
OBJECTIVE: It has been reported that women fare worse after ischemic coronary events, but the mechanisms remain unclear. Because platelets play a central role in the formation of occlusive thrombi at sites of ruptured atherosclerotic plaques, we studied male/female paired mouse littermates for sex differences in platelet function. METHODS AND RESULTS: We compared platelet reactivity in male/female mouse littermates by monitoring agonist-induced fibrinogen (FGN) binding and platelet aggregation. Compared with the platelets from males, platelets from females bound more FGN in response to low concentrations of thrombin and collagen-related peptide. Female platelets also demonstrated greater aggregation in response to adenosine diphosphate and collagen-related peptide. Platelet protein tyrosine phosphorylation on activation also showed small differences between sexes. These differences are independent of platelet size and surface expression of alphaIIbbeta3 and GPIb-IX-V, and they were not blocked by apyrase or aspirin. The sex differences we observed were intrinsic to platelets, because they were observed in washed platelets, but not when platelets were in plasma. CONCLUSIONS: The platelets of female mice were more reactive than those of males in a manner independent of COX-1 and secreted ADP.
Leng et al. (Tue,) conducted a other in Platelet reactivity. Female platelets vs. Male platelets was evaluated on Agonist-induced fibrinogen binding and platelet aggregation. Platelets from female mice demonstrated intrinsically greater reactivity, including increased fibrinogen binding and aggregation in response to agonists, compared with platelets from male mice.
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