Host angiotensin II type 1a receptor deficiency significantly reduced tumor capillary density and tumor growth compared to wild-type mice by impairing macrophage infiltration and VEGF expression.
The host angiotensin II type 1 receptor pathway promotes tumor-associated macrophage infiltration and VEGF expression, supporting tumor angiogenesis and growth in vivo.
Absolute Event Rate: 18% vs 37%
p-value: p=<0.05
Although the renin angiotensin system (RAS) is a major regulator of vascular homeostasis, the role of the RAS in tumor angiogenesis is little understood. Here we show that host angiotensin II (ATII) type 1 (AT1) receptor plays an important role in angiogenesis and growth of tumor cells engrafted in mice. Subcutaneous B16-F1 melanoma-induced angiogenesis as assessed by tissue capillary density and microangiography was prominent in WT mice but was reduced in AT1a receptor-deficient (AT1a-/-) mice. Consequently, tumor growth rate was significantly slower, and the mouse survival rate was greater, in AT1a-/- mice than in WT mice. Tumor growth was also reduced in WT mice treated with TCV-116, a selective blocker of AT1 receptor. Because the beta-galactosidase gene was inserted into the AT1a gene locus in AT1a-/- mice, the site of beta-galactosidase expression represents the AT1a receptor expression in these mutant mice. In tumor-implanted AT1a-/- mice, the major site of the beta-galactosidase expression was macrophages in tissues surrounding tumors. Moreover, the number of infiltrated macrophages was significantly lower in AT1a-/- mice than in WT mice, and double-immunofluorescence staining revealed that these macrophages expressed VEGF protein intensively. Therefore, the host ATII-AT1 receptor pathway supports tumor-associated macrophage infiltration, which results in enhanced tissue VEGF protein levels. The host ATII-AT1 receptor pathway thereby plays important roles in tumor-related angiogenesis and growth in vivo.
Egami et al. (Tue,) conducted a other in Tumor angiogenesis and growth. AT1a receptor deficiency / TCV-116 vs. Wild-type (WT) control was evaluated on Tumor capillary density (CD31-positive vessels per field) (p=<0.05). Host angiotensin II type 1a receptor deficiency significantly reduced tumor capillary density and tumor growth compared to wild-type mice by impairing macrophage infiltration and VEGF expression.