Key points are not available for this paper at this time.
262 INTRODUCTION 262 THE OOLGI APPARATUS, TON AND PCR 263 The Plant Golgi Apparatus Consists of a Set of Dispersed, Functionally Independent Golgi Stack-TGN Units 263 A Goigi Matrix Encompasses E a c h Golgi Stack and its Trans-Golgi Network ... 264 Cryofixed Golgi Stacks: Differences in Cisternae and Stack Architecture ..... 266 TON vs PCR: Two Functional Domains of the Same Organelle? 268 The Biosynthesis of N-Glycans Involves Enzymes in ER and Golgi Compartments ... ........ 270 a-Glyc an Biosynthesis is Initiated in Cis-Golgi Cisternae ........ 271 Complex Polysaccharides are Assembled in Golgi Stacks and the TGN 272 Golgi Membrane Proteins are Targeted by Signals in their Transmembrane Domains, and TGN Proteins by Signals in their Cytoplasmic Tails ........ 274 VESICLE-MEDIATED TRANSPORT MECHANISMS 274 Transport Through the Golgi Stack: A Critical Evaluation of Models 274 The Vesicle Transport Machinery is Highly Conserved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .... . . .... 276 COP and Clathrin Vesicle Coats are Composed of Many Proteins .... ..... 278 Assembly of Coated Vesicles Involves Sarlp and ARF GTP-Binding Proteins 279 Vesicle Targeting Involves Vesicle-Specific Ligand-Receptor Systems ...... ........ 280 The Vesicle Fusion Machinery is Shared by Different Transport Systems....... 280 Rab GTP-Binding Proteins may be Proofreaders of vlt-SNARE Pair Formation, andlor ActivatorslDeactivators of the Fusion Complex . 281 CONCLUDING REMARKS ... .. 282 The Future? . . .. ........ ........ .... .... .... .... .... .... 282
Staehelin et al. (Thu,) studied this question.