Effect of read-mapping biases on detecting allele-specific expression from RNA-sequencing data

Key Points

• To evaluate how read-mapping biases influence the detection of allele-specific expression in RNA-sequencing data.
• Analysis of RNA-sequencing datasets
• Assessment of different read-mapping techniques
• Comparison of allele-specific expression results under varying mapping conditions
• Read-mapping biases were identified to significantly affect gene expression estimates.
• Different mapping strategies led to varying detection rates of allele-specific expression.
• Impacts of biases indicate potential inaccuracies in RNA-sequencing data interpretation.