The CSF RT-PCR assay demonstrated a sensitivity of 77.8% and specificity of 100% for detecting enteroviral meningitis, compared to a sensitivity of 66.7% for viral culture alone.
Cohort (n=90)
Yes
Does a CSF RT-PCR assay improve the sensitivity and specificity of diagnosing enteroviral meningitis compared to viral culture in pediatric patients?
The RT-PCR assay provides a rapid and reliable method with high specificity and improved sensitivity over viral culture for detecting enteroviral meningitis in children.
Absolute Event Rate: 77.8% vs 66.7%
OBJECTIVE: To assess the sensitivity and specificity of a new polymerase chain reaction (PCR) assay with uninterrupted reverse transcription and complementary DNA amplification (RT-PCR) for the diagnosis of enteroviral (EV) meningitis in children. DESIGN: A prospective, cohort study. SETTINGS: Two medical centers: 1 university hospital and 1 children's hospital in San Diego County, California, during a 5-week period. PATIENTS: All pediatric patients younger than 16 years who underwent a lumbar puncture for evaluation of possible meningitis. MAIN OUTCOME MEASURES: The results of cerebrospinal fluid (CSF) RT-PCR were compared with viral cultures and clinical histories. RESULTS: During the 5-week period, 90 patients were entered into the study. Nonpolio EVs were cultured from 10% (9/90) of the patients from the following sites: CSF, 6.7% (6/90) of the patients; stool, 19% (4/21) of the patients; and throat swabs, 5.6% (1/18) of the patients. The EV genome was detected in the CSF by using RT-PCR in 7 of 9 EV culture-positive patients. The sensitivity and specificity of the CSF RT-PCR assay to detect EV meningitis were 77.8% and 100%, respectively. This compared with a sensitivity of 66.7% for detection of EV in CSF by viral culture alone. CONCLUSION: The new RT-PCR assay is a rapid and reliable method for the detection of EV infection in childhood.
Ronn E. Tanel (Sun,) conducted a cohort in enteroviral meningitis (n=90). CSF RT-PCR assay vs. viral culture was evaluated on sensitivity to detect enteroviral meningitis. The CSF RT-PCR assay demonstrated a sensitivity of 77.8% and specificity of 100% for detecting enteroviral meningitis, compared to a sensitivity of 66.7% for viral culture alone.