What question did this study set out to answer?

This research aims to identify a T cell growth factor and understand its interaction with the interleukin-2 receptor.

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May 13, 1994

Cloning of a T Cell Growth Factor that Interacts with the β Chain of the Interleukin-2 Receptor

Key Points

This research aims to identify a T cell growth factor and understand its interaction with the interleukin-2 receptor.
Isolated a complementary DNA clone for a new cytokine, identified and designated as interleukin-15.
Analyzed the biological activity of IL-15 in stimulating T lymphocyte proliferation.
Used monoclonal antibodies to examine the receptor interactions of IL-15 with IL-2.
IL-15 significantly stimulates the proliferation of T lymphocytes.
Monoclonal antibodies targeting the beta chain of the IL-2 receptor inhibited the action of IL-15.
IL-15 competes with IL-2 for binding to the IL-2 receptor, suggesting shared receptor components.

Abstract

A cytokine was identified that stimulated the proliferation of T lymphocytes, and a complementary DNA clone encoding this new T cell growth factor was isolated. The cytokine, designated interleukin-15 (IL-15), is produced by a wide variety of cells and tissues and shares many biological properties with IL-2. Monoclonal antibodies to the beta chain of the IL-2 receptor inhibited the biological activity of IL-15, and IL-15 competed for binding with IL-2, indicating that IL-15 uses components of the IL-2 receptor.

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