The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine increased P-selectin-positive platelets from 2.5% to 5.1% (P<0.05), demonstrating platelet-leukocyte cross talk.
Platelet-leukocyte cross talk in whole blood involves multiple mediators including PAF, superoxide anion, 5-lipoxygenase products, integrins, and selectins.
Absolute Event Rate: 5.1% vs 2.5%
p-value: p=<0.05
Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditions: at 37 degrees C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N:-formyl-methionyl-leucyl-phenylalanine (10(-6) mol/L) increased P-selectin-positive platelets from 2.5+/-0. 1% to 5.1+/-0.6% (P:<0.05). The increase was inhibited by either the platelet-activating factor (PAF) antagonist SR27417, the superoxide anion scavenger superoxide dismutase, the 5-lipoxygenase inhibitor Zileuton, or the 5-lipoxygenase-activating protein inhibitor MK-886, suggesting the involvement of PAF, superoxide anion, and 5-lipoxygenase products in leukocyte-induced platelet activation. The platelet-specific agonist collagen (1 microg/mL) increased leukocyte CD11b expression from 2.94+/-0.52 to 3.81+/-0.58 (P:<0. 05); this was not inhibited by the thromboxane A(2) receptor antagonist ICI 192.605 or the PAF antagonist SR27417. Platelet P-selectin expression induced by N:-formyl-methionyl-leucyl-phenylalanine and leukocyte CD11b expression induced by collagen could be suppressed by glycoprotein IIb/IIIa blockade or P-selectin blockade. This study documents platelet-leukocyte cross talk under conditions that mimic a physiological state and suggests that this involves multiple mediators and mechanisms. Furthermore, new evidence of integrin and selectin involvement in intracellular and intercellular signaling during platelet-leukocyte cross talk is provided.
Li et al. (Fri,) reported a other. N-formyl-methionyl-leucyl-phenylalanine vs. Baseline was evaluated on Platelet P-selectin expression (p=<0.05). The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine increased P-selectin-positive platelets from 2.5% to 5.1% (P<0.05), demonstrating platelet-leukocyte cross talk.