An ethanol-acetic acid-formol saline fixative for routine use with special application to the fixation of non-perfused rat lung

An ethanol-acetic acid-formol saline fixative (40 : 5 : 10 : 45 v/v) has been developed which gives good results with non-perfused rat lung and which may be used routinely for the fixation of a wide range of rat tissues. The special qualities of the fixative include good penetration, good fixation of nuclei and mitotic chromosomes, and little shrinkage after paraffin embedding. The fixative is also easy to use and has a flexible fixation period (nominally 48 h). Although several fixative mixtures containing alcohol, acetic acid and formalin have previously been reported, none are identical to the present mixture, which was developed independently and systematically in accordance with specific listed requirements.