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Summary Methods of preparing intact, undenatured sheets of human stratum corneum are described. The epidermis of split or full-thickness skin is first separated by exposure to ammonia fumes or heat at 60 C. The horny layer is then isolated by incubating in dilute trypsin solution. Cantharidin blisters provide a convenient way of obtaining stratum corneum from living subjects with minimum discomfort and no scarring.
Albert M. Kligman (Sun,) studied this question.