Catching Bubbles: Targeting Ultrasound Microbubbles Using Bioorthogonal Inverse‐Electron‐Demand Diels–Alder Reactions

Catching bubbles: Tetrazine-functionalized microbubbles were prepared for use as ultrasound contrast agents, and shown to selectively localize on cells labeled with a TCO-derivatized antibody against VEGFR2. This capture approach based on labeling and bioorthogonal chemistry was validated in a flow-chamber assay and in vivo through ultrasound imaging of VEGFR2-positive and VEGFR2-negative murine tumor models. TCO=trans-cyclooctene. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.