Knockdown of the cellular protein hnRNP D with siRNA significantly hampers Hepatitis C virus infection and inhibits HCV IRES-dependent translation.
The cellular protein hnRNP D plays a crucial role in HCV IRES-dependent translation and viral proliferation, highlighting its potential importance in HCV infection.
Hepatitis C virus (HCV) is one of the major causative agents of virus-related hepatitis, liver cirrhosis, and hepatocellular carcinoma in humans. Translation of the HCV polyprotein is mediated by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the genome. Here, we report that a cellular protein, hnRNP D, interacts with the 5' border of HCV IRES (stem-loop II) and promotes translation of HCV mRNA. Overexpression of hnRNP D in mammalian cells enhances HCV IRES-dependent translation, whereas knockdown of hnRNP D with small interfering RNAs (siRNAs) inhibits translation. In addition, sequestration of hnRNP D with an interacting DNA oligomer inhibits the translation of HCV mRNA in an in vitro system. Ribosome profiling experiments reveal that HCV RNA is redistributed from heavy to light polysome fractions upon suppression of the hnRNP D level using specific siRNA. These results collectively suggest that hnRNP D plays an important role in the translation of HCV mRNA through interactions with the IRES. Moreover, knockdown of hnRNP D with siRNA significantly hampers infection by HCV. A potential role of hnRNP D in HCV proliferation is discussed.
Paek et al. (Thu,) conducted a other in Hepatitis C virus (HCV) infection. hnRNP D modulation (overexpression or siRNA knockdown) was evaluated on HCV IRES-dependent translation and HCV infection. Knockdown of the cellular protein hnRNP D with siRNA significantly hampers Hepatitis C virus infection and inhibits HCV IRES-dependent translation.