Renal failure (uremia) was not associated with increased cTnT or CK-MB content in exterior oblique muscle compared to healthy controls (detected in 47% vs 40% of samples, respectively).
Observational (n=24)
Does uremia affect the content of cTnT or CK-MB in exterior oblique muscle in patients with renal failure compared to healthy controls?
Uremia does not increase cTnT or CK-MB content in exterior oblique muscle, suggesting that elevated serum cTnT in renal failure patients does not originate from skeletal muscle.
Absolute Event Rate: 47% vs 40%
BACKGROUND: Serum cardiac troponin T (cTnT) concentrations may be increased in patients with renal dysfunction without evidence of cardiac damage, as assessed by conventional methods. It has been suggested that these positive measurements result from the expression in skeletal muscle of fetal isoforms of cTnT, which are detected by the cTnT immunoassay. METHODS: Skeletal muscle (exterior oblique) biopsies were taken from healthy living kidney donors (n = 5) and transplant recipients (n = 19). The amounts of cTnT and creatine kinase (CK) isoenzymes in skeletal muscle of healthy controls were compared with those in patients with renal failure (Wilcoxon-Mann-Whitney test). cTnT was measured quantitatively by a second-generation assay, with a limit of detection of 1 microg/g of protein, and qualitatively by immunohistochemistry and immunoblotting. CK-MB was measured by quantitative electrophoresis. RESULTS: Minute quantities of cTnT were detected in 2 of the 5 (40%) control samples and 9 of the 19 (47%) renal failure samples, respectively, at mean concentrations of <5 microg/g of protein for both subject groups. This was <1/6000th that found in heart muscle. There was no significant difference in cTnT or CK-MB content in skeletal muscle between healthy controls and patients with renal failure. Increased serum cTnT did not predict detectable cTnT in skeletal muscle. cTnT was not detected qualitatively by immunoblotting or immunohistochemistry in any skeletal muscle samples. CONCLUSIONS: Uremia does not affect the content of cTnT or CK-MB in exterior oblique muscle, suggesting that cTnT detected in serum from patients with renal failure does not originate from skeletal muscle.
Fredericks et al. (2001) conducted an observational in Renal failure (n=24). Renal failure (uremia) vs. Healthy controls was evaluated on Detection of cTnT in skeletal muscle. Renal failure (uremia) was not associated with increased cTnT or CK-MB content in exterior oblique muscle compared to healthy controls (detected in 47% vs 40% of samples, respectively).