The WB-DSA Western blot method was evaluated for the early detection of cardiac troponin I in 6 patients presenting with chest pain.
Case Report (n=6)
Does the WB-DSA method detect Cardiac Troponin I early after onset of chest pain in patients?
A Western blot method (WB-DSA) may allow for earlier detection and characterization of cardiac troponin I degradation patterns in patients presenting with chest pain.
Patients presenting to the emergency departments (ED) with symptoms of acute coronary syndrome (ACS) and with a nondiagnostic electrocardiogram (ECG) pose a management challenge (1). Cardiac troponins (cTns), tropinin I (cTnI) and tropinin T [cTnT), creatine kinase (CK), and CK-MB are frequently used in the assessment of ACS. cTns are superior in their analytical specificity and diagnostic sensitivity and specificity for myocardial injury (2)(3). Findings from both animal and clinical studies show that cTnI is released into the blood in various cardiac conditions, including angina, acute myocardial infarction (1)(4)(5), congestive heart failure (6), and myocarditis (7). Because cTns in serum represent myocardial damage and increased risk of future adverse outcomes (8), improving the detection of serum cTns has implications for better diagnosis of myocardial damage and better risk stratification for patients with ACS. With current clinical assays, cTns are detectable in the circulation 4–6 h after the onset of pain in acute myocardial infarction, peaking within 12–24 h and remaining increased for a few days (9). However, a recently developed Western blot method, WB-DSA (10), detected minute amounts of cTnI in serum of patients undergoing bypass surgery within 10 min after reperfusion (11), suggesting increased detection of TnI by the WB-DSA method. Although WB-DSA does not permit analysis of troponin’s quaternary structure, it does allow accurate assessment of the chemical status of individual troponin subunits, such as the extent and pattern of cTnI degradation. cTnI is specifically degraded in ischemic/reperfused injured rat myocardium (4)(12), and TnI degradation products are detected in myocardium of patients undergoing coronary artery bypass surgery. Because ACS represents a spectrum of cardiac pathophysiology, unique patterns of cTnI degradation may be present in serum at various points along this spectrum and detectable by the WB-DSA. This study presents a …
Colantonio et al. (Mon,) conducted a case report in Chest pain (n=6). WB-DSA Western blot method was evaluated. The WB-DSA Western blot method was evaluated for the early detection of cardiac troponin I in 6 patients presenting with chest pain.