Obesity and cardiac lipid accumulation in mouse models and cultured myocytes were associated with a 36-72% decrease in ventricular ANP and BNP expression.
Obesity and altered cardiac lipid metabolism are associated with reduced production of ANP and BNP in the cardiac ventricles, providing a potential mechanism for the clinical observation of reduced plasma natriuretic peptides in obese individuals.
Effect estimate: 36-72% decrease
Plasma B-type natriuretic peptide (BNP) and proBNP are established markers of cardiac dysfunction. Even though obesity increases the risk of cardiovascular disease, obese individuals have reduced plasma concentrations of natriuretic peptides. The underlying mechanism is not established. We used cultured cardiomyocytes and three different mouse models to examine the impact of obesity and cardiac lipid accumulation on cardiac natriuretic peptide expression. The cardiac ventricular expression of atrial natriuretic peptide (ANP) and BNP mRNA and ANP peptide was decreased 36-72% in obese ob/ob, db/db, and fat-fed C57BL/6 mice as compared with their respective controls. The db/db and ob/ob mice displayed impaired cardiac function, whereas the fat-fed mice had almost normal cardiac function. Moreover, the ventricular expression of hypertrophic genes (α- and β-myosin heavy chain and α-actin) and natriuretic peptide receptor genes were not consistently altered by obesity across the three mouse models. In contrast, cardiac ventricular triglycerides were similarly increased by 60-115% in all three obese mouse models and incubation with oleic acid caused triglyceride accumulation and an approximately 35% (P < 0.005) depression of ANP mRNA expression in cultured HL-1 atrial myocytes. The data suggest that obesity and altered cardiac lipid metabolism are associated with reduced production of ANP and BNP in the cardiac ventricles in the setting of normal as well as impaired cardiac function.
Bartels et al. (Wed,) conducted a other in Obesity and cardiac lipid accumulation. Obesity and cardiac lipid accumulation vs. Respective controls was evaluated on Cardiac ventricular expression of ANP and BNP mRNA and ANP peptide (36-72% decrease). Obesity and cardiac lipid accumulation in mouse models and cultured myocytes were associated with a 36-72% decrease in ventricular ANP and BNP expression.