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November 1, 2015Open Access

Isolation and Functional Analysis of Human Neutrophils

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Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.

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Authors

Douglas B. Kuhns

Leidos (United States)

Debra A. Long Priel

Leidos (United States)

Jessica Chu

Codexis (United States)

Journals

Current Protocols in Immunology

Actions

Institutions

National Institutes of Health

National Institute of Allergy and Infectious Diseases

Frederick National Laboratory for Cancer Research

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Kuhns et al. (Sun,) studied this question.

synapsesocial.com/papers/6a00c9cf2ff633f3657817bd — DOI: https://doi.org/10.1002/0471142735.im0723s111

Also consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Neutrophil Methods and Protocols· 2007 · 70 citations
Neutrophils and immunity: challenges and opportunities· 2006 · 2,923 citations
Toll-Like Receptors· 2009 · 2 citations
Editorial: Will the real neutrophil please stand up?· 2011 · 18 citations
Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.· 1985 · 781 citations

Also consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Neutrophil Methods and Protocols· 2007 · 70 citations
Neutrophils and immunity: challenges and opportunities· 2006 · 2,923 citations
Toll-Like Receptors· 2009 · 2 citations
Editorial: Will the real neutrophil please stand up?· 2011 · 18 citations
Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.· 1985 · 781 citations

Isolation and Functional Analysis of Human Neutrophils

Key Points

Abstract

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Discussion

Authors

Journals

Actions

Institutions

References and Citations

Citation Network

Connected Papers

Discussion

Cite this study

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