Use of Polyethylene Glycol to Separate Free and Antibody-Bound Peptide Hormones in Radioimmunoassays†

Key Points

• This research aims to develop a method for separating free and antibody-bound peptide hormones in radioimmunoassays using polyethylene glycol.
• Developed a separation method using aqueous polyethylene glycol for immunoassays.
• Applied the method to radioimmunoassays of insulin, parathyroid hormone, growth hormone, and arginine vasopressin.
• Compared this method with the traditional double-antibody precipitation method.
• Successfully separated antibody-bound peptide hormones with minimal effect on free hormones.
• Demonstrated improved accuracy in measuring low molecular weight substances compared to traditional methods.

Abstract