Simple Automated Determination of Serum or Plasma Glucose by a Hexokinase/Glucose-6-Phosphate Dehydrogenase Method

Abstract A simple, fast, efficient, and accurate automated method is described for determining the quantity of glucose in 50 µl of serum or plasma. A hexokinase/glucose-6-phosphate dehydrogenase reaction requiring incubation at room temperature is used. The method is specific for glucose, and is not affected by increased concentrations of endogenous chemical substances in uremic sera or by several common drugs that interfere with other glucose methods. Results of parallel assays, in which glucose oxidase and o-toluidine are used, are compared.