A boundary anomaly found in the ultracentrifugal sedimentation of mixtures

Key Points

• Investigate anomalous boundary behavior occurring during the analytical ultracentrifugal sedimentation of multicomponent mixtures.
• Analyzed the sedimentation velocity profiles of macromolecular mixtures in an analytical ultracentrifuge.
• Tracked boundary formation, displacement rates, and concentration gradients across mixed solute systems.
• Identified marked deviations in boundary patterns when solutes sediment together compared to when they sediment individually.
• Observed that inter-particle hydrodynamic and Johnston-Ogston-type effects alter the apparent concentration and migration rates of mixture components.