Enzymatic Synthesis of 1,6-Digalloylglucose from β-Glucogallin by β-Glucogallin: β-Glucogallin 6-O-Galloyltransferase from Oak Leaves

Abstract Cell-free extracts from oak (Quercus robur) leaves catalyze the transfer of the galloyl-moiety of β-glucogallin (1-O-galloyl-β-D-glucopyranose) specifically to the 6-position of the same compound, yielding 1,6-di-O-galloyl-β-D-glucopyranose, an intermediate of gallotannin biosynthesis, β- Glucogallin thus functions as both donor and acceptor molecule in this reaction. The partial purification and some general properties of this new acyltransferase are reported.