The TraDIS toolkit: sequencing and analysis for dense transposon mutant libraries

Barquist et al. (Thu,) studied this question.

www.synapsesocial.com/papers/69deab13afb501b9b655857f — DOI: https://doi.org/10.1093/bioinformatics/btw022

Also consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data· 2011 · 1,251 citations
2. Splinkerettes—improved vectorettes for greater efficiency in PCR walking· 1995 · 205 citations
3. Simultaneous assay of every <i>Salmonella</i> Typhi gene using one million transposon mutants· 2009 · 654 citations
4. <tt>edgeR</tt> : a Bioconductor package for differential expression analysis of digital gene expression data· 2009 · 44,213 citations
5. TRANSIT - A Software Tool for Himar1 TnSeq Analysis· 2015 · 284 citations