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Under the particular experimental conditions developed and herein described, the production of a yellow color by dispersed HeLa cell cultures in a medium containing phenol red, is regularly prevented by un-neutralized poliomyelitis virus. Thus, a simplified method is available for titration of the poliomyelitis viruses and their type-specific antibodies.
Lipton et al. (1955) studied this question. A simplified colorimetric test using HeLa cell cultures and phenol red enables the titration of poliomyelitis viruses and their type-specific antibodies.