The Relation between the Minor Components of Whole Normal Human Adult Hemoglobin as Isolated by Chromatography and Starch Block Electrophoresis

Three hemoglobin components may be separated from hemolysates of normal adult red blood cells by starch block electrophoresis: the main component, A₁, moves at a position intermediate between the most slowly moving component, A₂, and the most rapidly moving component, A₃. By chromatography, eight hemoglobin components may be detected: five (A_ (Ia), A_ (Ib), A_ (Ic), A_ (Id) and A_ (Ie) ) move down the column more rapidly than the main component (An) and two move more slowly (Ania and Amb). Non-heme containing proteins are also present. The present investigation has determined the correspondence the components as isolated by the two methods: AI contains AI (c), A_ (Id) and A_ (Ie), and A_ (II) ; A₂ contains _ (IIIb) and non-heme protein (s) ; A₃ contains A_ (Ia), A (Ib) and non-heme protein (s).