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Three novel strains of the phylum Acidobacteria (Ac₁1E3T, Ac₁2G8T and Ac₁6C4T) were isolated from Namibian semiarid savanna soils by a high-throughput cultivation approach using low-nutrient growth media. 16S rRNA gene sequence analysis placed all three strains in the order Blastocatellales of the class Blastocatellia (Acidobacteria subdivision 4). However, 16S rRNA gene sequence similarities to their closest relative Pyrinomonas methylaliphatogenes K22T were ≤90 %. Cells of strains Ac₁1E3T, Ac₁2G8T and Ac₁6C4T were Gram-staining-negative and non-motile and divided by binary fission. Ac₁1E3T and Ac₁6C4T formed white colonies, while those of Ac₁2G8T were orange-yellowish. All three strains were aerobic chemoorganoheterotrophic mesophiles with a broad pH range for growth. All strains used a very limited spectrum of carbon and energy sources for growth, with a preference for complex proteinaceous substrates. The major respiratory quinone was MK-8. The major shared fatty acid was iso-C15: 0. The DNA G+C contents of strains Ac₁1E3T, Ac₁2G8T and Ac₁6C4T were 55. 9 mol%, 66. 9 mol% and 54. 7 mol%, respectively. Based on these characteristics, the two novel genera Brevitaleagen. nov. and Arenimicrobiumgen. nov. are proposed, harboring the novel species Brevitaleaaridisoli sp. nov. (Ac₁1E3T=DSM 27934T=LMG 28618T), Brevitalea deliciosa sp. nov. (Ac₁6C4T=DSM 29892T=LMG 28995T) and Arenimicrobium luteum sp. nov. (Ac₁2G8T=DSM 26556T=LMG 29166T), respectively. Since these novel genera are only distantly related to established families, we propose the novel family Pyrinomonadaceaefam. nov. that accommodates the proposed genera and the genus Pyrinomonas (Crowe et al. , 2014).
Wüst et al. (Thu,) studied this question.