What is the clinical evidence from this study?

Study design: RCT. Population: Healthy volunteers (n=30). Intervention: Fat + Glucose meal vs. Fat meal (75 g of fat). Primary outcome: Total GLP-1 AUC (0-2 h) [pmol*h/l] (p=<0.001).

What does this research mean for the field?

Adding 25 g of glucose to a 75 g fat load surprisingly reduces the total and active GLP-1 response by approximately 22% compared to a fat load alone, while stimulating a faster chylomicron response without affecting overall postprandial triglyceridemia. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

November 18, 2015Open Access

The Effect of Glucose When Added to a Fat Load on the Response of Glucagon-Like Peptide-1 (GLP-1) and Apolipoprotein B-48 in the Postprandial Phase

Q: What are the key findings of this study?

The addition of 25 g of glucose to a 75 g fat load surprisingly reduced the total GLP-1 response by approximately 22% compared to a fat load alone.

Q: What does this research mean for the field?

Adding 25 g of glucose to a 75 g fat load surprisingly reduces the total and active GLP-1 response by approximately 22% compared to a fat load alone, while stimulating a faster chylomicron response without affecting overall postprandial triglyceridemia. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Key Result

The addition of 25 g of glucose to a 75 g fat load surprisingly reduced the total GLP-1 response by approximately 22% compared to a fat load alone.

Study Design

Type

RCT (n=30)

Randomization

Randomized crossover

Multicenter

Structured PICO

Does adding glucose to a fat load affect the response of GLP-1 and apolipoprotein B-48 in the postprandial phase in healthy men?

Population

30 healthy male volunteers

Intervention

Experimental meal containing 75 g of fat + 25 g of glucose (F+G meal)

Comparator

Experimental meal containing 75 g of fat (F meal)

Outcome

Response of total and active glucagon-like peptide-1 (GLP-1) concentration and apolipoprotein B-48 concentrations within 8 hsurrogate

Adding glucose to a fat load alters early postprandial GLP-1 and chylomicron responses but does not affect overall triglyceride-rich lipoprotein cholesterol or triglycerides over 8 hours.

Main Result

Absolute Event Rate: 28.4% vs 36.2%

p-value: p=<0.001

Limitations

Small sample size of only 30 participants
Study restricted to healthy young male volunteers, limiting generalizability
The exact mechanism of how glucose addition suppresses the GLP-1 response remains to be determined

Abstract

Increased and prolonged postprandial lipemia has been identified as a risk factor of cardiovascular disease. However, there is no consensus on how to test postprandial lipemia, especially with respect to the composition of an experimental meal. To address this question of how glucose, when added to a fat load, affects the selected parameters of postprandial lipemia, we carried out a study in 30 healthy male volunteers. Men consumed an experimental meal containing either 75 g of fat + 25 g of glucose (F+G meal) or 75 g of fat (F meal) in a control experiment. Blood was taken before the meal and at selected time points within the following 8 h. Glucose, when added to a fat load, induced an increase of glycemia and insulinemia and, surprisingly, a 20 % reduction in the response of both total and active glucagon-like peptide-1 (GLP-1) concentration. The addition of glucose did not affect the magnitude of postprandial triglyceridemia and TRL-C and TRL-TG concentrations but stimulated a faster response of chylomicrons to the test meal, evaluated by changes in apolipoprotein B-48 concentrations. The addition of glucose induced the physiological response of insulin and the lower response of GLP-1 to the test meal during the early postprandial phase, but had no effect on changes of TRL-cholesterol and TRL-TG within 8 h after the meal.

Mark Helpful

Bookmark

Relay

View Full Paper