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Our and other groups have discovered that mesenchymal stem cells (MSCs) derived exosomes are a novel therapeutical modality for many diseases. In this study, we summarized a method to extract and purify hucMSCs‐exosomes using ultrafiltration and gradient centrifugation in our laboratory and proved that hucMSCs‐exosomes prepared according to our procedure were stable and bioactive. Results showed that exosomes derived from hucMSC were 40~100 nm and CD9 and CD81 positive. Functionally, hucMSCs‐exosomes promoted cell proliferation and protected against oxidative stress‐induced cell apoptosis in vitro by activation of ERK1/2 and p38. Interestingly, UV exposure abrogated the regulatory roles of exosomes under oxidative stress, indicating that hucMSCs‐exosomes may regulate cell growth and apoptosis by exosomal shuttle of RNA. Furthermore, cytokine profile analysis revealed that hucMSCs‐exosomes contained high dose of IL‐6, IL‐8, and other cytokines. The established method is practical and efficient, which provides a basis for further evaluating the potential of hucMSCs‐exosomes as therapeutic agents.
Zhang et al. (Fri,) studied this question.