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// Yang Yang 1, * , Qin He 2, * , Hong Wang 1, * , Xinyue Hu 1 , Ying Luo 1 , Guojuan Liang 1 , Shengnan Kuang 1 , Shaoshan Mai 1 , Jie Ma 1 , Xiaoyan Tian 1 , Qi Chen 1 , Junqing Yang 1 1 Department of Pharmacology, Chongqing Medical University, The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China 2 Department of Hepatobiliary Surgery, 1st Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China * These authors contributed equally to this work Correspondence to: Junqing Yang, email: cqjqyang2004@aliyun.com , cqyangjq@cqmu.edu.cn Keywords: chronic aluminium load, liver function, inflammation, oxidative stress Received: December 28, 2016 Accepted: February 13, 2017 Published: February 21, 2017 ABSTRACT The present study was designed to observe the protective effect and mechanisms of meloxicam on liver injury caused by chronic aluminium exposure in rats. The histopathology was detected by hematoxylin-eosin staining. The levels of prostaglandin E2, cyclic adenosine monophosphate and inflammatory cytokines were detected by enzyme linked immunosorbent assay. The expressions of cyclooxygenases-2, prostaglandin E2 receptors and protein kinase A were measured by western blotting and immunohistochemistry. Our experimental results showed that aluminium overload significantly damaged the liver. Aluminium also significantly increased the expressions of cyclooxygenases-2, prostaglandin E2, cyclic adenosine monophosphate, protein kinase A and the prostaglandin E2 receptors (EP 1,2,4 ) and the levels of inflammation and oxidative stress, while significantly decreased the EP3 expression in liver. The administration of meloxicam significantly improved the impairment of liver. The contents of prostaglandin E2 and cyclic adenosine monophosphate were significantly decreased by administration of meloxicam. The administration of meloxicam also significantly decreased the expressions of cyclooxygenases-2 and protein kinase A and the levels of inflammation and oxidative stress, while significantly increased the EP 1,2,3,4 expressions in rat liver. Our results suggested that the imbalance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway is involved in the injury of chronic aluminium-overload rat liver. The protective mechanism of meloxicam on aluminium-overload liver injury is attributed to reconstruct the balance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway.
Yang et al. (Tue,) studied this question.