Culture-independent deep sequencing of stool from vaccinated children detected no vaccine-derived polioviruses but identified low-level (<5%) and high-level (>90%) vaccine-related variants.
Cohort
Does a culture-independent deep-sequencing method reliably detect emerging vaccine-related poliovirus variants in stool samples of vaccinated children?
Deep sequencing of stool samples provides a reliable, culture-independent method to monitor emerging vaccine-related poliovirus variants, aiding global eradication efforts.
Oral poliovirus vaccine can mutate to regain neurovirulence. To date, evaluation of these mutations has been performed primarily on culture-enriched isolates by using conventional Sanger sequencing. We therefore developed a culture-independent, deep-sequencing method targeting the 5' untranslated region (UTR) and P1 genomic region to characterize vaccine-related poliovirus variants. Error analysis of the deep-sequencing method demonstrated reliable detection of poliovirus mutations at levels of 90%). These results suggest that monitoring emerging vaccine-related poliovirus variants by deep sequencing may aid in the poliovirus endgame and efforts to ensure global polio eradication.
Sahoo et al. (Thu,) conducted a cohort in Poliovirus vaccination. Culture-independent deep sequencing was evaluated on Detection of vaccine-derived polioviruses and variants. Culture-independent deep sequencing of stool from vaccinated children detected no vaccine-derived polioviruses but identified low-level (<5%) and high-level (>90%) vaccine-related variants.
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