Physiological 1 nM AngII enhanced the Ca2+ response, which was further amplified by calcium channel blockers (nifedipine and verapamil) in vascular smooth muscle cells and cardiomyocytes.
Calcium channel blockers amplify the AT1-receptor mediated calcium signaling response to physiological concentrations of Angiotensin II, suggesting a reciprocal regulation that may impact their use as monotherapy in hypertension.
Blockers of angiotensin II type 1 receptor (AT1R) and the voltage gated calcium channel 1.2 (CaV1.2) are commonly used for treatment of hypertension. Yet there is little information about the effect of physiological concentrations of angiotensin II (AngII) on AT1R signaling and whether there is a reciprocal regulation of AT1R signaling by CaV1.2. To elucidate these questions, we have studied the Ca2+ signaling response to physiological and pharmacological AngII doses in HEK293a cells, vascular smooth muscle cells and cardiomyocytes using a Ca2+ sensitive dye as the principal sensor. Intra-cellular calcium recordings were performed in presence and absence of CaV1.2 blockers. Semi-quantitative imaging methods were used to assess the plasma membrane expression of AT1R and G-protein activation. Repeated exposure to pharmacological (100 nM) concentrations of AngII caused, as expected, a down-regulation of the Ca2+ response. In contrast, repeated exposure to physiological (1 nM) AngII concentration resulted in an enhancement of the Ca2+ response. The up-regulation of the Ca2+ response to repeated 1 nM AngII doses and the down-regulation of the Ca2+ response to repeated 100 nM Angll doses were not accompanied by a parallel change of the AT1R plasma membrane expression. The Ca2+ response to 1 nM of AngII was amplified in the presence of therapeutic concentrations of the CaV1.2 blockers, nifedipine and verapamil, in vascular smooth muscle cells, cardiomyocytes and HEK293a cells. Amplification of the AT1R response was also observed following inhibition of the calcium permeable transient receptor potential cation channels, suggesting that the activity of AT1R is sensitive to calcium influx. Our findings have implications for the understanding of hyperactivity of the angiotensin system and for use of Ca2+ channel blockers as mono-therapy in hypertension.
Bernhem et al. (Wed,) conducted a other in Hypertension. Angiotensin II and CaV1.2 blockers (nifedipine, verapamil) vs. Absence of CaV1.2 blockers was evaluated on Ca2+ signaling response. Physiological 1 nM AngII enhanced the Ca2+ response, which was further amplified by calcium channel blockers (nifedipine and verapamil) in vascular smooth muscle cells and cardiomyocytes.