Pharmacological inhibition of PDE5 increased skeletal muscle blood flow and O2 uptake during exercise in older men before training (P<0.05), but these effects were absent after aerobic training.
Does aerobic high-intensity exercise training improve skeletal muscle blood flow and oxidative metabolism via enhanced cGMP signaling in older men?
Aerobic high-intensity exercise training improves skeletal muscle blood flow and oxygen delivery in older men by enhancing cyclic GMP signaling.
p-value: p=<0.05
Physical activity has the potential to offset age-related impairments in the regulation of blood flow and O 2 delivery to the exercising muscles; however, the mechanisms underlying this effect of physical activity remain poorly understood. The present study examined the role of cGMP in training-induced adaptations in the regulation of skeletal muscle blood flow and oxidative metabolism during exercise in aging humans. We measured leg hemodynamics and oxidative metabolism during exercise engaging the knee extensor muscles in young n = 15, 25 ± 1 (SE) yr and older ( n = 15, 72 ± 1 yr) subjects before and after a period of aerobic high-intensity exercise training. To determine the role of cGMP signaling, pharmacological inhibition of phosphodiesterase 5 (PDE5) was performed. Before training, inhibition of PDE5 increased ( P < 0.05) skeletal muscle blood flow and O 2 uptake during moderate-intensity exercise in the older group; however, these effects of PDE5 inhibition were not detected after training. These findings suggest a role for enhanced cGMP signaling in the training-induced improvement of regulation of blood flow in contracting skeletal muscle of older men. NEW & NOTEWORTHY The present study provides evidence for enhanced cyclic GMP signaling playing an essential role in the improved regulation of blood flow in contracting skeletal muscle of older men with aerobic exercise training.
Piil et al. (Thu,) conducted a other in Aging (n=30). Aerobic high-intensity exercise training and PDE5 inhibition vs. Before training was evaluated on Skeletal muscle blood flow and O2 uptake during moderate-intensity exercise (p=<0.05). Pharmacological inhibition of PDE5 increased skeletal muscle blood flow and O2 uptake during exercise in older men before training (P<0.05), but these effects were absent after aerobic training.